Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Plasma concentrations of leptin, oestradiol and progesterone in the six experimental groups examined in this study

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Clinical Proteomics

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: In this and subsequent figures (2, ​,44 and ​and5):5): (1) data from only the highest concentration of leptin (10 ng ml−1) are shown; (2) the horizontal bar indicates the period during which leptin (▴) or vehicle (ACSF, ○) was infused; (3) the time of perfusate collection in the upper three graphs is shifted 10 min ahead of the actual time of perfusion because the dead space of the pull system (150 μl) corresponds to a 10 min period of perfusion (flow rate, 15 μl min−1); (4) measurements of α-MSH, NPY and GnRH in the perfusates are expressed as point values at the centre of their collection periods; and (5) dotted lines in the graphs for α-MSH, NPY and GnRH indicate the limits of detection for each peptide. Number of rats in each subgroup = 8-10.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Concentration Assay

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Open bars, ACSF (control); bars with horizontal lines, leptin (1.0 ng ml−1); bars with vertical lines, leptin (3.0 ng ml−1); filled bars, leptin (10 ng ml−1).* Statistically significant vs. the ‘before’ values of the respective groups. † Statistically significant vs. the other three groups. ‡ Statistically significant vs. the leptin (1.0 ng ml−1) group.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Control

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Open bars, ACSF (control); bars with horizontal lines, leptin (1.0 ng ml−1); bars with vertical lines, leptin (3.0 ng ml−1); filled bars, leptin (10 ng ml−1). *Statistically significant vs. the ‘before’ values of the respective groups. † Statistically significant vs. the other three groups. ‡ Statistically significant vs. the leptin (1.0 ng ml−1) group. ** Statistically significant vs. the ACSF and leptin (1.0 ng ml−1) groups.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Control

Journal: Journal of Neuroinflammation

Article Title: Interleukin-6 and YKL-40 predicted recurrent stroke after ischemic stroke or TIA: analysis of 6 inflammation biomarkers in a prospective cohort study

doi: 10.1186/s12974-022-02467-1

Figure Lengend Snippet: Associations of individual inflammation marker or combination of IL-6 and YKL-40 with recurrent stroke within 1 year

Article Snippet: The concentrations of IL-6, IL-1Ra, Lp-PLA 2 and YKL-40 were determined by using enzyme-linked immunosorbent assay kits (catalogue number: PHS600C for IL-6, PDRA00B for IL-1Ra, DPLG70 for Lp-PLA 2 and DC3L10 for YKL-40, R&D Systems, Inc, Minneapolis, MN, USA).

Techniques: Marker

Journal: Journal of Neuroinflammation

Article Title: Interleukin-6 and YKL-40 predicted recurrent stroke after ischemic stroke or TIA: analysis of 6 inflammation biomarkers in a prospective cohort study

doi: 10.1186/s12974-022-02467-1

Figure Lengend Snippet: Associations of individual inflammation marker or combination of IL-6 and YKL-40 with a Modified Rankin Scale score ≥ 2 within 1 year

Article Snippet: The concentrations of IL-6, IL-1Ra, Lp-PLA 2 and YKL-40 were determined by using enzyme-linked immunosorbent assay kits (catalogue number: PHS600C for IL-6, PDRA00B for IL-1Ra, DPLG70 for Lp-PLA 2 and DC3L10 for YKL-40, R&D Systems, Inc, Minneapolis, MN, USA).

Techniques: Marker, Modification

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 1. Representative cytokines secreted from isolated SOL in response to 1 mg/mL LPS dissolved in Krebs buffer (n ¼ 8 per group), Stats are Wilcoxon Signed ranks for matched pairs *P < 0.05, ** P < 0.01. T0 ¼ the beginning of LPS exposure, T1 ¼ 1 h after LPS exposure, T2 ¼ 2 h after LPS.

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Isolation

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 2. Representative cytokine secretory responses in isolated mouse SOL and mouse extensor digitorum longus (EDL) in response to 1 mg/mL LPS in Krebs buffer þ 1% serum. Stats used Wilcoxon- Signed ranks for matched pairs from time 0 to T1, and T1 to T2. ** ¼ P < 0.01, *** P < 0.001, n ¼ 10 for SOL, n ¼ 17 for EDL. T0 ¼ the beginning of LPS exposure, T1 ¼ 1 h after LPS exposure, T2 ¼ 2 h after LPS.

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Isolation

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 4. Comparisons of total cytokine secretion (log scale) of SOL vs. EDL at T2. Muscles were incubated in Krebs þ 1% plasma. T2 ¼ accumulated cytokines at the end of the 2 h exposure to LPS. Means SD, n ¼ 11 for SOL, n ¼ 16 for EDL. Comparisons are two sample t for parametric and Mann–Whitney for nonparametric samples. * ¼ P < 0.05, ** P < 0.01

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Muscles, Incubation, Clinical Proteomics, MANN-WHITNEY

Journal: Immunity

Article Title: The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis

doi: 10.1016/j.immuni.2018.08.016

Figure Lengend Snippet: Key Resources Table

Article Snippet: Recombinant Mouse LBP Protein , RD systems , 6635-LP-025/CF.

Techniques: In Vivo, Enzyme-linked Immunospot, Recombinant, Transfection, In Situ, Enzyme-linked Immunosorbent Assay, Selection, Control, Software

Journal: Nature Communications

Article Title: Tumor agnostic drug delivery with dynamic nanohydrogels

doi: 10.1038/s41467-025-66788-4

Figure Lengend Snippet: a Whole-body imaging of mice after intravenous injection of free siRNA (Cy3-labeled, 16 µg labeled siRNA) or Texas Red-labeled SANGs (1 mg•kg –1 , carrying 16 µg unlabeled siRNA). Tumor bioluminescence and SANGs fluorescence were imaged sequentially in ovarian cancer-bearing and control mice, showing distribution at 30 min and 24 h after i.v. injection. b Whole-body imaging of ovarian cancer-bearing mice 72 h after intravenous injection of SANGs (1 mg•kg –1 ) or saline control. Ex vivo imaging of tumors and major organs was immediately imaged after in vivo imaging was completed. c Quantification of SANG delivery and retention to ovarian tumors. d Quantification of SANG delivery and retention across specified time points across all major organ systems, free siRNA (Cy3-labeled) used as control ( n = 4–5 biological replicates). e , f Representative ex vivo images at 4 and 24 h after intravenous injection of SANGs (1 mg•kg –1 ) to mice following metastatic tumor induction. g Quantification of SANG delivery and retention to metastatic ovarian tumors, free siRNA (Cy3-labeled) used as quantitative control ( n = 4-8 biological replicates). h Colocalization analysis of ex vivo SANG fluorescence and tumor bioluminescence. Dotted line of identity indicates perfect colocalization with ±15% bounding conditions. Signals above this ±15% bounding conditions indicate off-target SANGs, i.e., dye-labeled SANGs fluorescence signal without corresponding bioluminescence signal. Below this ±15% bounding conditions show untargeted cancer, i.e., bioluminescence without a corresponding SANGs signal. i illustration of the study to compare SANG and LNP biodistribution created with BioRender. j Determination by flow cytometry of the efficiency of AlexaFluor-647 labeled siRNA against EGFR to CD31 − CD45 − , CD31 + CD45 − , and CD31 - CD45 cell populations from liver and kidneys by LNP-LP01 ( n = 3 biologically independent experiments) and SANGs ( n = 4 biologically independent experiments) relative to PBS (1X pH 7.4) control ( n = 3). All SANG fluorescence data were acquired with Texas Red-labeled particles. Barplots present data as mean ± se as well as all data points. (*) indicates statistically significant differences between experimental groups as empirically derived from a hierarchical Bayesian model (stan_glm): 95% highest density intervals do not overlap between groupwise contrasts.

Article Snippet: LNP-LP01 (designated LNP-INT01 in this reference ) were formulated in a Precision Nanosystems Ignite system by mixing siRNA against EGFR (Invitrogen 4390824) conjugated with Alexa Fluor 647 in citrate buffer with the stereopure ionizable lipid LP01, DMG-PEG2K (Avanti Lipids: #880151), cholesterol (Avanti Lipids #700100), and DSPC (Avanti Lipids #860365) in 100% ethanol.

Techniques: Imaging, Injection, Labeling, Fluorescence, Control, Saline, Ex Vivo, In Vivo Imaging, Flow Cytometry, Derivative Assay